Современная биоинженерия и решение актуальных проблем! Специально для вас выступают 4 спикера c 4 актуальными и очень интересными темами, которые касаются науки и перспектив биологических дисциплин.

While DNA sequencing is a useful tool for determining what's going on in a cell or a person's body, it only tells part of the story. Protein sequencing could soon give researchers a wider window into ...

Author summary Protein identification is the key step in many proteomics studies. Currently, the most popular technique for intact protein analysis is top-down mass spectrometry which recently enabled high-throughput identification of many proteins and their proteoforms. However, this approach requires large amounts of materials and is currently limited to short proteins, typically less than 30 kDa. On the other hand, nanopore sensors promise single molecule sensitivity in protein analysis, but an approach for the identification of a single protein from its blockade current (nanospectrum) has remained elusive, since the signal from the sensors relates to the amino acid sequence of the protein in a poorly understood way. In this work we describe the first algorithm for protein identification based on nanospectra associated with translocation of proteins through pores with sub-nanometer diameters. While identification accuracy currently does not allow reliable processing of complex protein samples yet, we believe…